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Introduction of a mutated site into the hexon gene in the HAdV-4 genome. Fragment H4A3R7 (here designated hexon-PCR), with a mutation in HVR7 of the HAdV-4 hexon region, was generated by overlapping PCR extension mutagenesis. The mutated fragment was then cloned into the MluI-digested pBRAd4 vector to generate the mutagenesis vector pBRAd4-A3R7 with homologous recombination in vitro by using Exnase II.
