FIG 8.

MCPyV ST expression reduces the phosphorylation levels of β₁ integrin at Thr788/789 residues. (A) (i) HEK-293 cells were transfected with 1 μg EGFP, EGFP-ST, EGFP-ST102A, or EGFP-ST103A. After 24 h, the cell lysates were probed for phosphorylated Thr788/789 residues of β₁ integrin. GAPDH was used to measure equal loading. 2T2 was used to probe for MCPyV ST expression. (ii) Densitometry quantification of the Western blots was carried out using Image J software and is shown as a percentage relative to the loading control, GAPDH. The data were analyzed using three replicates per experiment (n = 3), and statistical analysis was performed using a two-tailed t test with unequal variance. *, P < 0.01. (B) (i) HEK-293 cells were transfected with 1 μg EGFP or EGFP-ST in the absence or presence of the PP4C transdominant mutant, PP4-RL. After 24 h, the cell lysates were probed for phosphorylated Thr788/789 residues of β₁ integrin. GAPDH was used to measure equal loading. 2T2 was used to probe for MCPyV ST expression. (ii) Densitometry quantification of the Western blots was carried out using Image J software and is shown as a percentage relative to the loading control, GAPDH. The data were analyzed using three replicates per experiment (n = 3), and statistical analysis was done using a two-tailed t test with unequal variance. *, P < 0.01. The error bars indicate standard deviations.