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The changes in SDF-1 mRNA levels in the sensory epithelium of inner ear after dose and time-dependent treatment with furosemide and kanamycin were assessed by real-time RT-PCR. (a) The maximal increase in SDF-1 occurred at the dose of furosemide 130 mg/kg + kanamycin 550 mg/kg. (b) SDF-1 levels increased significantly (more than 2.5-fold) on the 7th day after treatment with furosemide 130 mg/kg + kanamycin 550 compared with that of the control (P < 0.01).
