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Verification of the gene expression by qRT-PCR. Nine differentially expressed genes (DEGs) containing up- or downregulated genes in microarray analysis at each time point (3 and 10 dpi) were selected randomly for validation of the RNA-seq data. Gene expression in ECTV-infected C57BL/6 (a) and BALB/c (b) mice using qRT-PCR was analyzed using the 2−ΔΔCt method.
