Figure 3.

mRNA expression of the mitochondrially encoded complex I genes in the new born, P15, and adult lung tissues as determined via quantitative real-time PCR (qRT-PCR) analysis. Total RNA was extracted from the lung tissues of newborn (NB), P15, and adult (AL) mice using RNAzol, reverse transcribed and subjected to qRT-PCR using specific primers for the mRNAs for distinct subunits of the mitochondrially encoded complex I (mt-Nd1, mt-Nd2, mt-Nd3, mt-Nd4, mt-Nd4l, mt-Nd5, and mt-Nd6). The bar graphs represent relative levels of the transcripts in three independent experiments. p values were calculated by the one-way ANOVA using Tukey's test. Statistical significance is indicated by ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001. The mRNA levels were normalized to the ones of GAPDH.