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. 2017 Dec 19;2017:9169146. doi: 10.1155/2017/9169146

Figure 4.

Figure 4

mRNA expression of the complex II–V genes in new born, P15, and adult lung tissues as determined by real-time PCR analyses. Total RNA was extracted from lung tissue of newborn (NB), P15, and adult (AL) mice using RNAzol, reverse transcribed and subjected to qRT-PCR using specific primers for mRNAs of (a) the nuclear-encoded succinate dehydrogenase complex II subunit D (Sdhd), (b) the mitochondrially encoded complex III (mt-Cytb), (c–e) the different complex IV subunits (mt-Co1, mt-Co2, and mt-Co3), and (f) the mitochondrially encoded complex V (mt-Atp6). The bar graphs represent relative levels of the transcripts in three independent experiments. p values were calculated by the one-way ANOVA using Tukey's test. Statistical significance is indicated by ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. The mRNA levels were normalized to the ones of GAPDH.