Figure 5.

mRNA expression of the mtDNA transcription machinery genes, mitochondrially encoded RNA, and mitochondrial DNA polymerase in the new born, P15, and adult lung tissues as determined via quantitative real-time (qRT-PCR) analysis. Total RNA was extracted from the lung tissues of newborn (NB), P15, and adult (AL) mice using RNAzol, reverse transcribed and subjected to qRT-PCR using specific primers of the mtDNA transcription machinery (Polrmt, Tfam, Tfb1m, and Tfb2m), mitochondrially encoded RNAs (mt-Rnr1 and mt-Rnr2), and mitochondrial DNA polymerase (Polg2) involved in mitochondrial DNA replication. The bar graphs represent relative levels of the transcripts in three independent experiments. Statistical significance is indicated by ^∗ p < 0.05, ^∗∗ p < 0.01, and ^∗∗∗ p < 0.001. The mRNA levels were normalized to the ones of GAPDH.