Table 1.
List of primary and secondary antibodies used in this study.
| Primary antibody against antigen | Symbol | Host | Company | Cat. number | Dilution (WB) | Dilution (IF) | Dilution (IEM) |
|---|---|---|---|---|---|---|---|
| Mitochondrial proteins | |||||||
| NADH:ubiquinone oxidoreductase core subunit 1 (complex I) | MT-ND1 | Rabbit, polyclonal | Abcam | ab74257 | 1 : 500 | — | — |
| Succinate dehydrogenase complex subunit D (complex II) | SDHD, CybS | Rabbit, polyclonal | Millipore | ABT110 | 1 : 3000 | — | 1 : 50 |
| OXPHOS complex III core 2 subunit (complex III) | UQCR2 | Mouse, monoclonal | Invitrogen | A11143 | — | 1 : 250 | — |
| OXPHOS complex IV subunit I/cytochrome c oxidase I (complex IV) | COX1, MT-CO1 | Mouse, monoclonal | Invitrogen | 459600 | 1 : 1000 | — | 1 : 100 |
| OXPHOS complex IV subunit II/cytochrome c oxidase II (complex IV) | COX2, MT-CO2 | Mouse, monoclonal | Invitrogen | A6404 | 1 : 1000 | — | — |
| ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (complex V) | ATP5b | Mouse, monoclonal | Life Technologies | A21351 | 1 : 3000 | — | — |
| ATP synthase 6 (complex V) | MT-ATP6E | Rabbit, polyclonal | Santa Cruz | Sc-20946 | — | — | 1 : 50 |
| Polymerase (DNA) gamma 2, accessory subunit | POLG2 | Mouse, polyclonal | Abcam | ab66961 | 1 : 1000 | — | — |
| Mitochondrial transcription factor A | TFAM | Goat, polyclonal | Santa Cruz | Sc-19050 | — | — | — |
| Cell-specific markers | |||||||
| Prosurfactant protein C | Pro-SP-C | Rabbit, polyclonal | Chemicon | ab3786 | — | 1 : 1000 | — |
| Club cell protein 10 (CC10) | CC10 | Rabbit, polyclonal | Santa Cruz | sc-25555 | — | 1 : 1000 | — |
| Loading control | |||||||
| Glyceraldehyde 3-phosphate dehydrogenase | GAPDH | Mouse, monoclonal | HyTest, Finland | 5G4 | 1 : 8000 | — | — |
| Secondary antibodies | |||||||
| Fab anti-rabbit IgG ultrasmall gold | — | Goat | Aurion | 800.255 | — | — | 1 : 400 |
| Fab anti-mouse IgG ultrasmall gold | Goat | Aurion | 800.266 | — | — | 1 : 400 | |
| Anti-rabbit-IgG Alexa Fluor 488 | — | Donkey | Life Technologies | A21206 | — | 1 : 1000 | — |
| Anti-mouse-IgG Alexa Fluor 555 | — | Donkey | Life Technologies | A31570 | — | 1 : 1000 | — |
| Anti-rabbit-IgG alkaline phosphatase conjugate | — | Goat, polyclonal | Sigma-Aldrich | A0545 | 1 : 20,000 | — | — |
| Anti-mouse-IgG alkaline phosphatase conjugate | — | Goat, polyclonal | Sigma-Aldrich | A3562 | 1 : 20,000 | — | — |
| Counterstaining of nuclei for immunofluorescence | |||||||
| Hoechst 33342 (1 μg/ml) | — | — | Life Technologies | 33342 | — | 1 : 1000 | — |
| TOTO®-3 iodide | — | — | Life Technologies | T-3604 | — | 1 : 1000 | — |