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. 2017 Oct 30;37(1):50–62. doi: 10.15252/embj.201796880

Figure EV3. SAMHD1 inhibits HIV‐1 following DNA damage.

Figure EV3

  1. MDM were transfected with control or pool of SAMHD1 (KD) siRNA and 3 days later treated with 5 μM ETO and infected in the presence of ETO with HIV‐1 BaL (3, 5, 12 ffu/cell). Cells were stained for intracellular p24, and the percentage of infected cells was quantified 48 h post‐infection by FACS (n = 3, mean ± s.e.m.).
  2. MDM were treated with 5 μM ETO for 18 h and co‐infected in the presence of ETO with HIV‐1 BaL (3, 5, 12 ffu/cell) and SIVmac virus‐like particles containing Vpx/Vpr (SIV VLP). Cells were stained for intracellular p24, and the percentage of infected cells was quantified 48 h post‐infection by FACS (n = 3, mean ± s.e.m.).
  3. MDM were transfected with control or pool of SAMHD1 (KD) siRNA and 3 days later treated with 5 μM ETO for 18 h and infected in the presence of ETO with VSV‐G HIV‐1 GFP. The percentage of infected cells was quantified by the automated cell‐imaging system Hermes WiScan and ImageJ 48 h post‐infection (n = 3, mean ± s.e.m.; **P‐value ≤ 0.01; (ns) non‐significant, paired t‐test).
  4. Cells from a representative donor were used for immunoblotting.