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. 2017 Oct 20;37(1):63–74. doi: 10.15252/embj.201797833

Figure 1. AlkCα and AlkCβ are specific for N3‐methyladenine (3mA).

Figure 1

  1. 3mA excision reaction catalyzed by AlkC.
  2. Phylogenetic tree of 779 AlkC (orange, green) and 764 AlkD (blue) protein sequences visualized using the iTOL web server (Letunic & Bork, 2011).
  3. Schematic of AlkC and AlkD protein domains.
  4. Release of 3mA (black bars) and 7mG (gray bars) from methylated genomic DNA after a 1‐h incubation with either HCl, no enzyme (mock), Bacillus cereus AlkD, or one of seven AlkC orthologs. Bc, Bacillus cereus; Ba, Bacillus anthracis; Bm, Bacillus mycoides; Bw, Bacillus weihenstephanensis; S21, Sphingobacterium sp. 21; Pf, Pseudomonas fluorescens; Sa, Streptomyces albus. Values are the mean ± SD (n = 3 for each). HCl and no‐enzyme controls provide upper and lower limits of 3mA and 7mG detection.