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. 2017 Oct 20;37(1):63–74. doi: 10.15252/embj.201797833

Figure 6. AlkC excises 3‐methylcytosine (3mC) and 1‐methyladenine (1mA) from DNA .

Figure 6

  • A
    Venn diagram of the numbers of bacterial species containing either AlkB (blue), AlkC (yellow), or both (green).
  • B, C
    Chemical structures and in vitro base excision of 3mC (B) and 1mA (C) from 25‐mer double‐stranded oligodeoxyribonucleotides. Representative denaturing electrophoresis gels show substrate (S) and product (P) after a 24‐h incubation with either no enzyme (mock), BcAlkD, BcAlkC (AlkCα), or PfAlkC (AlkCβ). Plots show quantified time courses from three experiments (values are mean ± SD).
  • D
    3mC may be repaired in bacteria by either AlkB‐catalyzed oxidative demethylation or AlkC‐catalyzed base excision. αKG, α‐ketoglutarate.