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. 2017 Sep 25;37(1):89–101. doi: 10.15252/embj.201796751

Figure 7. Sde2 processing in telomeric silencing and genome stability.

Figure 7

  1. Aberrant overexpression of telomeric transcripts in Sde2 mutant. Expression levels of telomeric transcripts tlh1, tlh2 and SPAC212.06c were quantitated by RT–qPCR assays in WT, and Δsde2 strain at 30°C and 37°C is normalized with act1.
  2. Telomeric silencing assay in Sde2 mutants. ura4 + gene inserted at the telomere in Δsde2 strain (Sugioka‐Sugiyama & Sugiyama, 2011) was used as a marker to assay telomeric silencing on indicated agar plates. Suppression of growth on –Ura plate indicates silencing of the reporter at the telomeres.
  3. Mitotic chromosome stability assay to assess genomic instability in Sde2 mutants. Percentage loss of chromosome 16 in Δsde2 strain (Sugioka‐Sugiyama & Sugiyama, 2011) transformed with Sde2 variants is plotted (n = total number of colonies counted). Processing‐defective and lysine mutants of Sde2 show elevated loss of the chromosome.

Source data are available online for this figure.