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. 2017 Oct 12;6(4):34. doi: 10.3390/cells6040034

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of the acetyl-T22 in human histone H3 by immune assays. (a) Specificity of the anti-H3T22ac antibody was demonstrated by dot blot assay using four synthetic peptides: H3T22, un-acetylated peptide KQLATKAAR; H3T22ac, acetylated peptide KQLATacKAAR; M8S253ac, acetylated peptide KSacPLTEPNFENKC; M8S71ac, acetylated peptide TSacITPSSQDICRICHCEGDC. 50 and 500 ng of the peptides were used as indicated; (b) Detection of H3T22ac in human histones from different cell lysates. HEK293T (lane 1) and HeLa (lane 2) cell lysates were resolved by SDS-PAGE and detected by western blot assay using the antibodies against H3 (bottom) and H3T22ac (top).