Figure 2.

Establishing the CD4/CD8 lineage of responding PBMC. Unseparated PBMC (open bars), PBMC of the same subject that have been depleted of CD8 cells (blue bars), or of CD4 cells (light blue bars) were tested at the same cell number (250,000) per well. The numbers of IFN-γ producing cells were measured by ELISPOT after the addition of the antigens specified on the x-axis. Percent response in the depleted PBMC fractions is shown relative to the unseparated PBMC. Note, for varicella, parainfluenza, mumps and influenza the light blue bars are barely/non visible due to the close to complete abrogation of Spot Forming Units (SFU) formation in CD4 cell-depleted PBMC.