Table 2.
Forward and reverse primer sequences used for collagens in the chick OFT. The ARBP and PGK-1 primer sequences were obtained from [38,39], respectively. Other primer sequences were designed by our group using Primer Designer software (San Diego State University Biological Workbench, San Diego, CA, USA; and Northwestern University Oligo Calc, Chicago, IL, USA).
| Gene | Forward Sequence | Reverse Sequence |
|---|---|---|
| COL1A1 | TGC TGT TGA TAG CAG CGA CT | GTG TCC TCG CAG ATC ACC TC |
| COL1A2 | TGG TAG GGT TGG GCC AAT CG | GCA CCT TGG TTG CCA GTG AC |
| COL3A1 | CTG GTT TCC GTG GCT TAC CC | GCC AGG CTT CCC ATC ACT TC |
| COL6A1 | TCG CCT ATT CCA AGG GAA AC | CTG CGA TGT GAT GAC CTA CG |
| COL6A2 | CTC GGC GCA ATG CTG AAC TC | ATG GCA GCC CGA TCT CCA AG |
| COL6A3 | CTC CAT CAA CCT GGG AAG AG | GGC CAC CTT TGT AGA TCA CC |
| COL14A1 | GTC CCG AGG AAG CGA TAA TG | AGG AGC AGA CAA GGG TAA CG |
| ARBP | CCT GTG ATG TGA CTG TGC | ACT TTG TCT CCG GTC TTA ATC |
| PGK-1 | AAA GTT CAG GAT AAG ATC CAG CTG | GCC ATC AGG TCC TTG ACA AT |