Table 7.
Major studies that have evaluated a diagnosis of Pneumocystis pneumonia (PCP) using real-time PCR directed at major surface glycoproteins (MSG) and mitochondrial large subunit (mtLSU) rRNA genes, in non-HIV immunocompromised patients. Adapted from Reid et al. [77].
| Study | Target Gene | Specimen (No.) | No. PCP Episodes | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) |
|---|---|---|---|---|---|---|---|
| Flori et al. [146] | mtLSU rRNA | BAL (173) | 11 | 100 | 87 | NA | NA |
| Dini et al. [155] | mtLSU rRNA | Respiratory tract (932) | 150 | 100 | 83 a | 78.1 a | 100 a |
| Hauser et al. [156] | mtLSU rRNA | Respiratory tract (110) BAL (101) | 14 | 93 | 90–91 | 59–65 | 98–99 |
| Alanio et al. [152] | mtLSU rRNA | BAL (163) IS (115) | 16 | 100 | 85.7 | 72.4 a | 100 |
| Flori et al. [146] | MSG | BAL (173) | 11 | 100 | 98.6 | 84.6 a | 100 a |
| Alvarez-Martinez et al. [157] | MSG | BAL, IS (213) | 111 | 100 | 90.2 a | 82 a | 100 a |
| Fillaux et al. [153] | MSG | BAL (400) | 66 | 100 | 90.5 | 47 | 100 |
| Chumpitazi et al. [151] | MSG | BAL (66) | 18 | 100 | 97.7 | 95.5 | 100 |
BAL: bronchoalveolar lavage; IS: induced sputum; NA: not available; NPV: negative predictive value; PPV: positive predictive value. a Calculated from data presented in a respective publication and in Chumpitazi et al. [151].