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Shh binding affinity of linear and macrocyclic L2 mimics. (a) Dose-response curves for direct binding of the recombinantly produced FLAG tag-fused peptides to plate-immobilized GST-Shh as determined using HRP-conjugated anti-FLAG antibody. (b) Sequences and KD values corresponding to macrocyclic L2 mimics and linear L2-based peptide. X = MDYKDDDDKGSGS-. The mutated positions in the evolved macrocyclic peptides compared to the initial cyclic peptide HL2-m1 are highlighted.
