Skip to main content
. Author manuscript; available in PMC: 2018 Sep 13.
Published in final edited form as: J Am Chem Soc. 2017 Aug 30;139(36):12559–12568. doi: 10.1021/jacs.7b06087

Figure 6.

Figure 6

HL2-m5-induced suppression of Hh pathway signaling. (a) Dose dependent inhibition of luciferase expression (FF/Ren ratio) in Shh-stimulated NIH3T3 cells containing a dual luciferase reporter system. (b) Restoration of Hedgehog pathway signaling upon addition of purmorphamine (5 μM) to cells treated with HL2-m5 (10 μM). (c) Relative transcriptional levels of Gli1, Gli2, and Ptch1 genes in Shh-stimulated NIH3T3 cells in the presence and in the absence of HL2-m5 (10 μM) as determined by real-time PCR. mRNA levels in unstimulated cells are included for comparison.