Table 1.
Reported IVFC methods for fluorescence study of circulating cells in vivo
| IVFC Technique | Reference(s) | Animal Model | Cell Line(s) | Fluorophore(s) |
|---|---|---|---|---|
| Microscopy IVFC | (Georgakoudi et al., 2004) | SCID mice, Copenhagen rat | Leukemia (MLL), prostate cancer (LNCaP) | DiD |
| (Novak et al., 2004) | BALB/c mice | Red blood cells (RBC), white blood cells (WBC) | DiD, Cy-Chrome-CD45 | |
| (Sipkins et al., 2005) | BALB/c, SCID mice | Leukemic (NALM-6) | DiD, DiR | |
| (Wei et al., 2005) | SCID mice | Prostate cancer (MatLyLu) | AF-647 | |
| (Lee et al., 2006) | BALB/c mice | T cells (CD4+) | DiD | |
| (Boutrus et al., 2007) | NOD/SCID mice | Breast cancer (SUM149), Hematopoietic stem cells (HSC) | GFP, PE, APC | |
| (Novak and Puoris’haag, 2007) | BALB/c mice | RBC, T-Cells | DiD, CellTracker | |
| (Fan et al., 2010) | C57BL/6 mice | T-cells | GFP, DsRED | |
| (Hwu et al., 2011) | SCID mice | Breast cancer (SUM1315, DU4475) | GFP, FITC, (red) autofluorescence | |
| (Li et al., 2011) | BALB/c, Slac-nu mice | LNCaP, PC-3, HCCLM3, liver cancer (Hep62) | DiD, GFP | |
| (Runnels et al., 2011) | SCID, BALB/c, Col2.3 Mice | Multiple myeloma (MM) | GFP, DiD, DiO | |
| (Fan et al., 2012) | BALB/c mice | Hepatocellular carcinoma (HCCLM3) | GFP | |
| (Golan et al., 2012) | Human | RBC, WBC | Intrinsic scatter | |
| (Aceto et al., 2014) | SCID Mice | Breast cancer (MDA-MB-231-LM) | DiD | |
| (Hui et al., 2014) | BALB/c mice | Cervical cancer (HeLa) | Fluorescent nanodiamond (FND) | |
| (Nedosekin et al., 2014) | Nu/nu mice | Mammary adenocarcinoma (MTLn3) | Dendra2 | |
| (Suo et al., 2015) | BALB/c Mice | Bone marrow cells, mesenchymal stem cells (MSC) | DiO, IR-780 EGFP | |
| (Yan et al., 2015) | BALB/c mice | HCC | GFP | |
| (Suo et al., 2017) | BALB/c mice | HCCLM3, prostate cancer (PC-3) | GFP | |
| Multi-photon (MP-IVFC) | (He et al., 2007) | BALB/c mice | Leukemia (L1210A) | DiD, AF488, GFP, FITC |
| (Zhong et al., 2008) | Nu/nu mice | T-Lymphocytes (Jurkat), cervical cancer (KB), peripheral mononuclear blood cells (PMBC) | CFSE, DeepRed | |
| (Tkaczyk et al., 2008; | Nu/nu, CD-1 mice | RBCs, breast cancer | DeepRed, DiD | |
| Tkaczyk and Tkaczyk, 2011) | (MCF-7, MDA-MB-435) | |||
| (Chang et al., 2010) | CD1 mice | Fibrosarcoma (MCA-207) | DiD, GFP | |
| (Li et al., 2010) | BALB/c, C57BL/6 mice | Leukocytes | Intrinsic Tryptophan | |
| (Zeng et al., 2014) | zebrafish | WBCs (neutrophils, macrophages) | Intrinsic NADH, GFP, DsRed | |
| Retinal flow cytometer (RFC) | (Alt et al., 2007) | BALB/c mice | Lymphocytes | DiD |
| Computer Vision IVFC (CV-IVFC) | (Markovic et al., 2013; Markovic et al., 2015) | Nu/nu mice | MM | DiD |
| Diffuse Fluorescence Flow Cytometry (DiFC) | (Zettergren et al., 2012b; Zettergren et al., 2012a; Pera et al., 2017) | Nu/nu mice | MM | DiD |
| (Pestana et al., 2013) | Nu/nu mice | MSC | DiD | |
| Hybrid Photoacoustic Fluorescence Flow Cytometry (PA-FFC) | (Nedosekin et al., 2013) | Nu/nu mice | Melanoma (B16F10, C8161), MTLn3, RBC | FITC, GFP, iRFP, ICG |
| (Galanzha and Zharov, 2013) | Nu/nu mouse | Melanoma (B16F10, HTB-65, SK-ML-7), ovarian carcinoma (CI81) | GFP | |
| (Nedosekin et al., 2014) | Nu/nu mice | MTLn3 | Dendra2 | |
| (Juratli et al., 2014) | Nu/nu mice | B16F10, MDA-MB-231 | GFP | |
| (Nolan et al., 2016) | Nu/nu mice | B16F10, MDA-MB-231, MTLn3 | GFP, Dendra2 | |
| (Cai et al., 2016) | C57BL/6 mice | Infected (malaria) RBCs | GFP | |
| (Nedosekin et al., 2017) | Nu/nu mice | MDA-MB-231, breast cancer (ZR-75-1) | GFP, Gold Nanoparticles |