Fig. 2.

The antioxidant NAC induces chick retina regeneration. (A–C, E, F) Histological analysis of chick eyes collected 7d PR treated with (A) NAC, (B) FGF2, (C) retinectomy + vector (PBS), (E) Vitamin C, or (F) XJB 5–131. Scale bar in F is 125 μm and applies to all. L: Lens; RPE: Retinal pigmented epithelium; ciliary regeneration (CR) and RPE transdifferentiation (TD). (D) Quantitative analysis of the mean level of regeneration for each treatment: NAC (n = 10), FGF2 (n = 10). Statistical analysis was performed using nonparametric Mann-Whitney-Wilcoxon tests for both CR and TD. There was no significant difference in the regeneration induced from the CM by NAC and FGF2 treatments (Wilcoxon S = 115.0, P = 0.4813), however, FGF2 treated eyes showed a significant increase in RPE TD when compared to NAC (Wilcoxon S = 138.0, *P = 0.0107).