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. 2018 Jan 4;8:1. doi: 10.1186/s13578-017-0198-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — LPS induced the EMT of colon cancer cells via expression of TLR4. a, b Real-time PCR was employed to observe the effect of LPS on the EMT level of C26 and HCT116 cells. c Western blot was used to detect the expression of Vimentin and E-cadherin in C26 cells. d The inhibitory effect of siRNA on TLR4 expression in C26 cells was examined by western blot. e Transwell assay was used to detect the metastasis of C26 cells with TLR4 siRNA treatment. f, g Real-time PCR was employed to observe the EMT level of C26 cells when exposed to LPS with or without the expression of TLR4. (*P<0.05, **P < 0.01, ***P < 0.001)