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. 2018 Jan 4;11:6. doi: 10.1186/s13071-017-2567-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Identification of the CPLCG protein CPLCG5 in leg extracts from Culex pipiens pallens. a Analysis of cuticle protein leg extracts from 3-day-old adult mosquitoes (n = 40) by gradient gel electrophoresis (4−20%). The 12.7 kDa band was excised, digested with trypsin, and analysed by LC-MS/MS. b Nucleotide and deduced amino acid sequences of CPLCG5. The protein band was excised from the gel, digested with trypsin, and resulting peptides were analysed by LC-MS/MS. Proteins with two matched peptides are highlighted in grey, and include N103-K119 and N121-K129. N1-K18 is a predicted signal peptide. c, d, Western blotting analysis of CPLCG5 revealing ~2.2-fold higher protein levels in the DR strain than the DS strain (t-test, t₍₄₎ = -4.917, P = 0.008; **P < 0.01)