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. 2018 Jan 3;18:13. doi: 10.1186/s12885-017-3954-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Phosphatidylserine exposure and membrane permeability of HeLa cells after I-CRP exposure. a Cell death was measured by flow cytometry through Annexin-V and PI staining in HeLa cells treated with different concentrations (0.75, 1.0, 1.25 1.5, 2 U/mL) of I-CRP for 24 h. The percentages refer to Annexin-V-positive/PI-negative or Annexin-V-positive/PI-positive staining analyzed by flowjo software. b Cells were treated and analyzed as in (A) and graphed