Fig. 3.

Time-dependent characteristics of cell death induced by I-CRP in HeLa cells. a Cell death was measured by trypan blue exclusion after I-CRP treatment (1.25 U/mL) for 16 h, 24 h, 48 h, and 72 h and was analyzed as a percentage of living cells and graphed. b Effect on cell viability after withdrawal of I-CRP (1.25 U/mL) administered for 16 h, 24 h, 48 h and 72 h, was measured by MTT assay after 24 h of withdrawal. The percentages refer to relative cell viability represented as % control (non-treated cell viability = 100%). c Changes in morphology of HeLa cells induced by treatment with I-CRP (1.25 U/mL) for 16 h, 24 h, 48 h and 72 h, observed in an inverted microscope (NIKON TS100) (20X)