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Enrichment of phage peptides from library by BEFV G1 protein biopanning
| Item | First round biopanning | Second round biopanning | Third round biopanning | Fourth round biopanning |
|---|---|---|---|---|
| G1(μg) | 10 | 7.5 | 5 | 2.5 |
| Concentration of Tween20 (v/v) | 0.1% | 0.25% | 0.5% | 0.5% |
| Input/PFU (pfu/ml) | 1 × 1011 | 1 × 1011 | 1 × 1011 | 1 × 1011 |
| Output/PFU (pfu/ml) | 3.7 × 106 | 5.2 × 107 | 8.7 × 107 | 7.2 × 108 |
| Recovery (Output/input) |
3.7 × 10−5 | 5.2 × 10−4 | 8.7 × 10−4 | 7.2 × 10−3 |
