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. 2018 Jan 4;15:1. doi: 10.1186/s12974-017-1027-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effect of Aβ on astrocytes and glia activation in mouse hippocampus. Immunoreactivity of glial fibrillary acidic protein (GFAP) (a) and Iba1 (b) was analysed by SDS-PAGE and Western blotting in mouse hippocampus 3 h and 1, 3, 7, 14, 21 and 35 days after icv Aβ (0.5 nmol) injection. Representative pictures were shown. Results of densitometric analysis were normalised to immunoreactivity of GAPDH, as a loading control. Results are presented as the mean ± SEM from four independent experiments (n = 4) per time point. *p < 0.05; **p < 0.01; ***p < 0.001 versus control using a one-way ANOVA followed by the Bonferroni test. n.d. not detected