Fig. 7.

The effect of Aβ oligomers on calcium homeostasis and free radical generation in SH-SY5Y and BV2 cells. Cytoplasmic calcium level in BV2 (a) and SH-SY5Y (b) cells was measured by using Fluo-4 during 6 min after treatment with 10 μM Aβ oligomers. Data represent the mean value ± SEM for three independent experiments. c Responses of Fluo-4 were quantitated by measuring the area under the curve (AUC) value. Data represent the mean value ± SEM for three independent experiments. *, **p < 0.05 and 0.01 compared to control using Student’s t test. d Intracellular free radical level in SH-SY5Y and BV2 cells was measured by DCF fluorescence after 3 h incubation with 10 μM Aβ oligomers. Data represent the mean value ± SEM for four independent experiments. *p < 0.05 compared to control, using Student’s t test