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. 2018 Jan 3;9:1. doi: 10.1186/s13100-017-0106-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Retrotransposition Assay. The retrotransposition plasmid (pPM016) was transfected into cells. LINE-1 mRNA is expressed and processed, removing the γ globin intron from GFP during RNA splicing. ORF1p and ORF2p are translated and bind to LINE-1 mRNA, forming ribonucleoprotein (RNP) particles. The RNP enters the nucleus, where LINE-1 mRNA is reverse transcribed into host DNA by Target Primed Reverse Transcription (TPRT). After retrotransposition, cells expressing functional GFP are quantified by flow cytometry