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Co-localization of ORF2p and γH2AX (a) or PCNA (b) in LNCaP cells. Double immunofluorescence was performed using antibodies against ORF2 (red) and γH2AX (green) or PCNA (green). Cells were counterstained with DAPI. Images were taken on a Nikon TI Eclipse confocal microscope under 20× magnification and a representative maximum-intensity projection is shown. Arrowheads point to overlap of γH2AX and ORF2 (a) or PCNA and ORF2 (b) in the merged images
