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. 2017 Jan 3;20(1):97–98. doi: 10.4103/1008-682X.195561

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Copyright: © The Author(s)(2017)

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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(a) HE staining of testicular biopsies, showed a severe disruption in spermatogenesis. Arrows indicate the germ cells. Scale bars = 50 μm (left) and 20 μm (right). (b) Wet preparation of testicular biopsies, showed a Trichomonas-like flagellate (arrow). Scale bar = 20 μm. (c) Wright-Giemsa staining of the wet preparation smear, arrow indicates Trichomonas vaginalis. Scale bar = 25 μm. (d) PCR analysis of Trichomonas vaginalis from the semen and urine. A normozoospermic semen (normal semen) sample was applied as negative control. M: 100 bp DNA ladder. HE: Hematoxylin-Eosin; PCR: polymerase chain reaction.