Figure 3. GLAST+ astrocytes isolated from IL-1β treated mice induce Cox2 (Ptgs2) and express markers of “A2” reactivity.
Transcriptional analysis of cells isolated by magnetic bead purification from P5 mice treated with PBS or IL-1β A. Transcriptional induction of Cox2 in GLAST+ astrocytes and CD11b+ microglia isolated. B. Expression of pan-reactive markers (Gfap, Cxcl10) in GLAST+ astrocytes. C. Expression of A1-associated markers (Fbln5, Amigo2, Serping1) in GLAST+ astrocytes. D. Expression of A2- associated markers (Ptgs2, S100a10, Emp1, Cd109, Sphk1) in GLAST+ astrocytes. Data representative of n=13 per group. * p<0.05, ** p<0.01, **** p<0.001; analysis by Mann-Whitney test.