Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used for genotype screening of Cre and R26R carriers
| Gene | Primers | Band Size |
|---|---|---|
| Cre | P1: 5′-tgaccccatctcttcactcc-3′ P2: 5′-aactccacgaccacctcatc-3′ P3: 5′-agtccctcacatcctcaggtt-3 |
Wt: 225 bps Mutant: 225 bps + 287 bps |
| R26R | P1: 5′- gcgaagagtttgtcctcaacc-3′; P2: 5′- aaagtcgctctgagttgttat-3′ P3: 5′- ggagcgggagaaatggatatg-3 |
Wt: 650 bps Mutant: 300 bps |
