Table 1.
Experiment | DFMO (mM) | Spermidine nmoles/mg Protein | Spermine nmoles/mg Protein | Total Polyamines nmoles/mg Protein | Spd/Spm Ratio |
---|---|---|---|---|---|
Control | 0 | 13.0 ± 1.6 | 10.1 ± 0.9 | 23.1 | 1.29 |
DFMO | 0.1 | 12.0 ± 2.8 | 13.4 ± 4.3 | 25.3 | 0.90 |
DFMO | 0.5 | 0.7 ± 0.1 | 15.3 ± 2.3 | 16.1 | 0.05 |
DFMO | 1 | 0.2 ± 0.0 | 14.9 ± 0.8 | 15.2 | 0.01 |
DFMO | 2 | 0.6 ± 0.7 | 9.2 ± 3.9 | 9.8 | 0.06 |
DFMO | 3 | 0.1 ± 0.0 | 6.1 ± 0.2 | 6.2 | 0.01 |
DFMO | 4.2 | 0.2 ± 0.0 | 7.7 ± 1.0 | 7.9 | 0.02 |
a error is listed as standard deviation around the mean. Note the putrescine level for the control was 0.8 ± 0.6 nmoles/mg protein and was undetectable after DFMO addition.