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. 2017 Dec 28;23:6150–6161. doi: 10.12659/MSM.907377

Figure 4.

Figure 4

Berberine activated adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) in mouse liver tissue. (A, C) Mice were treated with 50, 100, and 200 mg/kg of berberine for three days. After treatment with berberine for 8 hours, Western blot analysis was used to detect the phosphorylation of acetyl coenzyme-A carboxylase (ACC). (B, D) Con A-injected mice were pretreated with 100 mg/kg berberine, with or without the adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) inhibitor, Compound C (20 mg/kg) for three days. At 8 hours after Con A injection, ACC phosphorylation levels were detected by Western blot analysis. The western blot bands were semi-quantified with NIH Image J software and normalized with internal control, β-actin (C, D) (n=6). ** P<0.01 vs. the control group, ## P<0.01 vs. the Con A group, §§ P<0.01 vs. the Con A + berberine group.