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. Author manuscript; available in PMC: 2018 Jan 4.
Published in final edited form as: Cell Rep. 2017 Dec 26;21(13):3717–3727. doi: 10.1016/j.celrep.2017.11.110

Figure 1. Real-time observation of Cascade target searching and binding by smFRET.

Figure 1

(A) Schematic of Cascade labeled with Cy5 in the Cas5e subunit. (B) Schematic of smFRET experiment using dsDNAtarget and targeting Cascade. (C–D) A representative smFRET time trajectory of (C) long-lived or (D) short-lived FRET events for targeting Cascade and dsDNAtarget. (E) Schematic of smFRET experiment using dsDNAtrunc and targeting Cascade. (F) A representative smFRET time trajectory of long-lived FRET for targeting Cascade and dsDNAtrunc. (G) Transition density plot of long-lived FRET events for targeting Cascade and dsDNAtarget with a FRET peak centered at 0.51. (H) Transition density plot of short-lived FRET events for targeting Cascade and dsDNAtarget with a FRET peak centered at 0.46. (I) Transition density plot of all FRET events for targeting Cascade and dsDNAtrunc with a FRET peak centered at 0.52. See also Figure S1–S4 and Table S3.