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. 2017 Nov 15;208(1):191–205. doi: 10.1534/genetics.117.300518

Figure 2.

Figure 2

TFIID is required for PIC formation at the PHO84 core promoter to initiate transcription in the absence of Pi in the growth medium. (A) ChIP analysis of Rpb1 at the PHO84 core promoter in the taf13-ts mutant and WT strains in the growth medium with or without Pi. (B) RT-PCR analysis of PHO84 mRNA and 18S rRNA levels in the WT and taf13-ts mutant strains in the growth medium without Pi. (C) RT-PCR analysis of PHO84 mRNA and 18S rRNA levels in the WT and taf13-ts mutant strains in the growth medium with Pi. (D) ChIP analysis for the recruitment of TAF1 to the PHO84 UAS, core promoter, and Chr.-V in the absence of Pi in the growth medium. Mock ChIP control was performed without using an antibody. (E) ChIP analysis for the recruitment of TAF10 and TAF12 to the PHO84 core promoter and Chr.-V in the absence of Pi in the growth media. (F) ChIP analysis for the recruitment of TAF10 and TAF12 to the PHO84 core promoter, PHO84 UAS, and Chr.-V in the absence and presence of Pi in the growth. (G) ChIP analysis for the recruitment of TAF1 to the PHO84 core promoter, PHO84 UAS, and Chr.-V in the presence of Pi in the growth medium. ChIP, chromatin immunoprecipitation; Chr.-V, chromosome-V; Pi, inorganic phosphate; UAS, upstream activating sequence; WT, wild-type.