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. 2017 Nov 15;208(1):191–205. doi: 10.1534/genetics.117.300518

Figure 3.

Figure 3

NuA4 HAT is required for PIC formation at the PHO84 core promoter (and hence transcription) in the absence of Pi in the growth medium. (A and B) ChIP analysis of Esa1 at the PHO84 UAS in the presence and absence of Pi in the growth media. (C) ChIP analysis of TBP and Rpb1 at the PHO84 core promoter in the esa1-ts mutant and WT strains in the presence or absence of Pi in the growth medium. (D) Esa1 is required for recruitment of a TFIID-specific TAF1 to the PHO84 core promoter in the absence of Pi in the growth medium. (E) RT-PCR analysis of PHO84 mRNA and 18S rRNA levels in the WT and esa1-ts mutant strains in the absence of Pi in the growth medium. (F) RT-PCR analysis of PHO84 mRNA and 18S rRNA levels in the WT and esa1-ts mutant strains in the presence of Pi in the growth medium. ChIP, chromatin immunoprecipitation; Chr.-V, chromosome-V; Pi, inorganic phosphate; PIC, preinitiation complex; UAS, upstream activating sequence; WT, wild-type.