The 19S RP is predominantly recruited to the PHO84 UAS to promote the targeting of NuA4 HAT and SAGA to the PHO84 UAS in the absence and presence of Pi in the growth media, respectively. (A and B) ChIP analysis of Rpt2 at the PHO84 promoter and an inactive region within Chr.-V in the growth medium with or without Pi. (C) ChIP analysis of NuA4 HAT at the PHO84 UAS in the WT and rpt4-ts mutant strains in the growth medium lacking Pi. (D) ChIP analysis of TAF10 and TAF12 at the PHO84 UAS in the WT and rpt4-ts mutant strains in the growth medium containing Pi. ChIP, chromatin immunoprecipitation; Chr.-V, chromosome-V; Pi, inorganic phosphate; RP, regulatory particle; UAS, upstream activating sequence; WT, wild-type.