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. 2017 Nov 15;208(1):191–205. doi: 10.1534/genetics.117.300518

Figure 6.

Figure 6

ChIP analysis of Pho2 and Pho4 at the PHO84 UAS in the presence and absence of Pi in the growth media. (A and B) ChIP analysis of Pho2 and Pho4 at the PHO84 UAS in the rpt4-ts mutant and WT strains in the growth medium lacking Pi. Maximum ChIP signal was set to 100 and other ChIP signals were normalized with respect to 100. (C) ChIP analysis of Pho2 at the PHO84 UAS in the Δspt20 and esa1-ts mutants and their isogenic WT equivalents in the absence of Pi in the growth medium. Statistical analysis was performed using four sets of biologically independent experiments. (D and E) ChIP analysis of Pho2 and Pho4 at the PHO84 UAS in the rpt4-ts mutant and WT strains in the growth medium containing Pi. (F) ChIP analysis of Pho2 at the PHO84 UAS in the Δspt20 and esa1-ts mutants and their isogenic WT strains in the presence of Pi in the growth medium. (G) ChIP analysis of Pho4 at the PHO84 UAS in the Δspt20 mutant and its isogenic WT equivalent in the growth medium with Pi. ChIP, chromatin immunoprecipitation; Chr.-V, chromosome-V; Pi, inorganic phosphate; UAS, upstream activating sequence; WT, wild-type.