ChIP analysis of RNA polymerase II recruitment at the PHO5 and PHO12 core promoters in the esa1-ts, taf13-ts, and rpt4-ts mutants and their isogenic WT equivalents in the absence of Pi in the growth medium. (A) Esa1 is required for TAF1 recruitment to the PHO5 and PHO12 core promoters in the growth medium lacking Pi. (B–D) TAF13, Esa1, and Rpt4 are required for recruitment of Rpb1 to the PHO5 and PHO12 core promoters in the growth medium without Pi. ChIP, chromatin immunoprecipitation; Pi, inorganic phosphate; WT, wild-type.