Table 3. H3.3^K9R and H3^K9R mutations are synthetically lethal.

	Embryo hatching				Pupatea				Eclose
Genotype	Obs	No.	%	Pb	Obs	No.	%	P	Obs	No.	%	P
H3HWT	389	450	86.4	—	98	140	70.0	—	88	140	62.9	—
H3K9R	370	480	77.1	< 0.0005	183	285	64.2	< 0.05	3	285	1.1	< 0.0005
H3.3K9R H3HWT	350	465	75.3	< 0.0005	279	462	60.4	< 0.0005	160	462	34.6	< 0.0005
H3.3K9R H3K9R	214	325	65.8	< 0.0005	0	130	0.0	< 0.0005	0	130	0.0	< 0.0005

Obs, observed; No., number; —, P value not determined.

^aThe pupation and eclosion values have an identical number of animals analyzed for each genotype because they were obtained from the same brood of animals, while the embryo hatching values were obtained from independent experiments.

^bP-value calculated with a χ² test using H3^HWT observed values as expected values.