Figure 3.

Deletion 3′ to ISM1 deletes regulatory information. CTCF binding sites (K562 blood cell line, dark blue) indicative of an insulator element (HMEC and NHEK epithelial cell lines, teal) are deleted by the 3′ ISM1 deletion (red). In addition, the deletion removes chromatin marks H3K4Me1 and H3K27Ac (indicative of enhancers, both shown in purple), which were also detected within the epithelial cell lines (orange, strong enhancer; yellow, weak enhancer; gray, heterochromatin; green, weak transcription; K562, human erythroleukemia cell line; HMEC, human mammary epithelial cells; NHEK, normal human epidermal keratinocytes).