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Knockdown of ism1 results in whole-embryo and craniofacial defects, which are rescued by ism1 mRNA. Whole-embryo knockdown of ism1 with 12 ng translation blocking (ATG) morpholino (MO) results in first branchial arch-derivative defects and is rescued with the injection of 200 pg ism1 mRNA lacking the MO binding site (P ≤ 0.001, Fisher’s Exact Test).
