Figure 7.

Expression of lhx8 decreases with knockdown of ism1. Embryos are shown in an anterior view with the cement gland (cg) labeled for ventral orientation. Control uninjected (Un) embryos or embryos pricked with the injection needle on half (HP) show strong lhx8 expression in the first branchial arch at stage 28 (A–D) surrounding the primitive mouth (yellow arrowhead), as well as in the maxillary prominences (green arrowhead). Knockdown of ism1 with 12 ng ATG morpholino (MO) in half of the animal results in undetectable (E) or decreased (F) branchial arch expression of lhx8, especially in the maxillary prominence.