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. 2017 Dec 18;26(10):1703–1715. doi: 10.1177/0963689717735112

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4. — Release of bilirubin, expressed as percentage of original bilirubin concentration, through a 20 kDa dialysis membrane, suspended in dialysate with or without protein. Group 1: nanoparticle bilirubin (nBR) in phosphate-bufferd solution (PBS) + 10% albumin; Group 2: free bilirubin (fBR) in PBS + 10% albumin; Group 3: nBR in PBS; Group 4: fBR in PBS. Area under the curve for bilirubin release in each of the 4 groups was significantly different (P = 0.0156). Release was significantly greater for nBR than for fBR and in solutions containing protein compared to PBS alone (P < 0.001). For nBR in PBS +10% FBS, there was an initial burst of release over approximately 8 h, followed by a more steady release up to 48 h.