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Shown are the results of HX-MS for (Panel A) V1C7, (Panel B) V1C7G29R, (Panel C) V5C1 (Panel D) V5C1R29G mapped onto the solvent accessible surface of RTA (PDB: 3SRP). Strong protection is colored dark blue, intermediate protection light blue, and intermediate deprotection light yellow, as defined in the Materials and Methods section and Supplemental Figure S4.
