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. Author manuscript; available in PMC: 2018 Jul 19.
Published in final edited form as: Neuron. 2017 Jul 6;95(2):341–356.e6. doi: 10.1016/j.neuron.2017.06.020

Figure 1. Microglial density varies significantly across basal ganglia (BG) nuclei and correlates with local abundance of astrocytes.

Figure 1

A – Coronal brain sections from P60 CX3CR1EGFP/+ mouse immunostained for tyrosine hydroxylase (TH), which labels dopamine neuron somas and projections. Yellow boxes indicate location of analyzed images. NAc = nucleus accumbens, VTA = ventral tegmental area, SNc = substantia nigra pars compacta, SNr = substantia nigra pars reticulata. B, C –Overlap between microglial marker Iba1 and EGFP in CX3CR1EGFP/+ mice; image from NAc. D – Distribution and density of EGFP+ BG microglia. ANOVA, F(3,17) = 508, P < 0.00001. N = 4–6 mice per region. E – Distribution and density of BG oligodendrocyte precursor cells (OPCs) immunostained for NG2. ANOVA F(3,20) = 0.14, P = 0.93 (n.s.). N = 6 mice per region. F – Distribution and density of BG neurons immunostained for NeuN. ANOVA F(3,8) = 108.8, P < 0.00001. N = 3 mice per region. G – Distribution and density of BG astrocytes in Aldh1l1-EGFP mice. ANOVA F(3,8) = 38.4, P = 0.00005. N = 3 mice per region. H – Left, ratio of EGFP+ microglia to NG2+ OPCs (ANOVA F(3,20) = 41.5, P < 0.00001); Middle, ratio of EGFP+ microglia to NeuN+ neurons (ANOVA F(3,8) = 33.9, P = 0.00007); Right, ratio of Iba1+ microglia to EGFP+ astrocytes (ANOVA F(3,8) = 1.7, P = 0.25, n.s.). Dashed yellow lines indicate SNc boundary. All mice were age P58–60. # P < 0.05 vs. NAc, ● P < 0.05 vs. VTA, † P < 0.05 vs. SNc, ¥ P < 0.05 vs. SNr, * P < 0.002 all individual comparisons.