Figure 3.

Treatment with ME reduces leukocyte infiltration and elevated protein in aqueous humor in LPS induced EIU. (A–C) Serial sections of paraformaldehyde-fixed rat eyes were stained with H&E and observed under bright field microscopy. C, cornea; CB, ciliary body; L. Lens: ×200 magnification. A representative photomicrograph is shown from each experimental group. (A) Control with PBS eye drops. (B) EIU 24 h with PBS eye drops. (C) EIU 24 hour with ME eye drops. (D, E) Leukocyte infiltration and protein concentration in aqueous humor. (D) Amelioration of LPS-induced inflammatory cell infiltration in aqueous humor by topical ME treatment. The inflammatory cells in aqueous humor were counted by the trypan blue exclusion method. (E) Reduction of total protein concentration in the aqueous humor as measured by Bradford method. *P < 0.05 versus CTR. #P < 0.05 versus LPS. ME treatment did not totally abolish the leukocyte infiltration especially protein leakage, suggesting dose adjustment or combinatorial therapy.