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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cancer Res. 2017 Oct 24;78(1):230–245. doi: 10.1158/0008-5472.CAN-17-1961

Fig. 2. Human oral MSCs are highly susceptible for KSHV infection and viral latent infection leads to morphological and cell marker changes of MSCs.

Fig. 2

(A) Primary oral MSCs of different origins (PDLSCs, GMSCs and DPSCs) were infected with GFP-KSHV in an MOI of 50 (KSHV genome equivalent) for 48 hours and analyzed by GFP fluoresces. (B) Infected cells were drug-selected for a week followed by two weeks culture without selection and analyzed by IFA with anti-Vimentin antibody. Images show the phase-contrast, the antibody staining and GFP fluorescence of the cells. (C) Flow cytometric analysis of mock- and KSHV-infected PDLSCs with mesenchyme markers (CD29, CD73, CD90, VD105 and CD166, Y-axis) and GFP (KSHV-infected cells, X-axis). (D) The expression of mesenchymal and endothelial markers in mock- and KSHV-infected PDLSCs.