Fig. 2.

Analysis of residual T cells after blocking T cell entry into LNs and Peyer’s Patches. a–c 6–12-week-old C57BL/6 Foxp3-GFP mice were injected or not i.p. with 200 μg of anti-LFA-1 (αL) and anti-VLA-4 (α4) Abs. Twenty-four hours later, cLNs, mLNs, Pp, and spleen were harvested and analyzed. a Diagram illustrating the experimental model. b Distribution of Treg, Tmem, and Tnaive cells among CD4 T cells in the indicated SLOs of treated or untreated mice. c Distribution of Treg, Tmem, and Tnaive cells among CD8 T cells in the indicated SLOs of treated or untreated mice. d, e 6–12-week-old C57BL/6 Foxp3-GFP mice were injected or not i.p. with 200 μg of anti-LFA-1 (αL) and anti-VLA-4 (α4) Abs every 2 days from day 0 to day 6 and SLOs were recovered for analysis at various time-points. d Experimental model. e Percentages of Treg, Tmem, and Tnaive cells among CD4 T cells are shown as means ± SEM for the indicated SLOs. f Relative absolute numbers of CD4 Treg, Tmem, and Tnaive cells in cLNs, mLNs, and spleen are shown. The percentage of recovery was calculated by dividing the absolute numbers in treated mice by the mean absolute number obtained in untreated animals. Data are means ± SEM for at least three independent experiments. Mouse clip arts were generated in ref. ²¹